A analysis staff led by Prof. Yang Liangbao from the Hefei Institutes of Bodily Science of the Chinese language Academy of Sciences has used surface-enhanced Raman spectroscopy (SERS) to precisely monitor the diffusion habits of a single molecule within the sub-nanometer house.
The examine was revealed in The Journal of Bodily Chemistry Letters.
SERS expertise, a extremely delicate and selective analytical approach, allows single-molecule-level evaluation by inducing a resonance phenomenon on a steel floor that considerably enhances the molecular Raman sign. Nonetheless, long-term monitoring of unlabeled single molecules stays a problem.
On this examine, the researchers used the superb photothermal impact of gold nanorods to assemble hotspot buildings with a spot dimension of ~ 1.0 nm utilizing laser reconstruction.
The constructed hotspot not solely supplied wonderful SERS enhancement but additionally actively trapped the goal molecules, enabling real-time monitoring and evaluation of the diffusion habits of crystalline violet single molecules.
“This allowed us to watch the blinking habits of single crystalline violet molecules for durations of as much as 4 minutes utilizing dynamic surface-enhanced Raman spectroscopy,” mentioned Yan Wuwen, a member of staff.
Combining density useful idea (DFT) calculations and SERS mapping outcomes, they concluded that the only crystalline violet molecules will be confined in sub-nanometer house.
This examine offers a singular solution to perceive molecular interactions, chemical reactions, and the habits of biomolecules.
Wuwen Yan et al, Actual-Time Monitoring of a Single Molecule in Sub-nanometer Area by Dynamic Floor-Enhanced Raman Spectroscopy, The Journal of Bodily Chemistry Letters (2023). DOI: 10.1021/acs.jpclett.3c02276
Chinese language Academy of Sciences
Scientists use SERS expertise to precisely monitor single-molecule diffusion habits (2023, October 11)
retrieved 12 October 2023
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